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cpg-b oligodeoxynucleotide-2006  (Kaneka Corp)

 
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    Kaneka Corp cpg-b oligodeoxynucleotide-2006
    Cpg B Oligodeoxynucleotide 2006, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpg-b+oligodeoxynucleotide-2006/phosphorothioate+stabilized+cpg+2006+oligonucleotide/pmc06436717-66-22-25
    Average 90 stars, based on 1 article reviews
    cpg-b oligodeoxynucleotide-2006 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients
    Article Snippet: To detect IL-10-producing Breg, 1x10 6 PBMCs were stimulated with CD40L (Alexis Biochemical; 200ng/ml, Barcelona, Spain), LPS (Sigma-Aldrich; 1μg/ml, Darmstadt, Germany) and CpG-B oligodeoxynucleotide-2006 (CpG; Eurogentec SA; 10μg/ml, Seraing, Belgium) for two days.

    Article Title: Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro
    Article Snippet: We treated B-cells with CD40L (eBioscience, San Diego, CA, USA; 200 ng/ml)+IL-4 (ImmunoTools, Friesoythe, Germany; 20 ng/ml), which induces B-cell differentiation, or with CpG-B oligodeoxynucleotide-2006 (CpG; Eurogentec SA, Liege, Belgium; 10 μg/ml)+CD40L+LPS (Sigma Aldrich; 1 μg/ml), which induces IL-10-producing Breg cells and a Breg phenotype in vitro .



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    Cryopreserved and thawed PBMC were ml in flat-bottom plates in AIM V medium with 10% human AB serum for 18 hours with cultured at 5×10 6 / 3 µg/ml CpG-ODN type B-2006-G5 at 37 ° C, 5% CO 2 . PMA (50 ng/ml) and ionomycin (750 ng/ml) were added after 12 hours. Brefeldin and monensin (PTI)(3 µg/ml and 2 µM, respectively) were added after 14 hours. PBMCs were stained for viability (yellow amine dye) prior to surface epitope and intracellular cytokine staining. Cytokine quadrants were set based on isotype controls.

    Journal: medRxiv

    Article Title: IFNβ-1b treatment leads to changes in the B cell subset and cytokine secretion profile in patients with relapsing-remitting multiple sclerosis

    doi: 10.1101/2022.02.25.22270266

    Figure Lengend Snippet: Cryopreserved and thawed PBMC were ml in flat-bottom plates in AIM V medium with 10% human AB serum for 18 hours with cultured at 5×10 6 / 3 µg/ml CpG-ODN type B-2006-G5 at 37 ° C, 5% CO 2 . PMA (50 ng/ml) and ionomycin (750 ng/ml) were added after 12 hours. Brefeldin and monensin (PTI)(3 µg/ml and 2 µM, respectively) were added after 14 hours. PBMCs were stained for viability (yellow amine dye) prior to surface epitope and intracellular cytokine staining. Cytokine quadrants were set based on isotype controls.

    Article Snippet: Cryopreserved and thawed PBMC (for both patients and healthy controls) were cultured at 5×10 6 /ml in 12-well, flat-bottom plates in AIM V medium supplemented with 10% pooled human AB serum for 18 hours with 3 µg/ml CpG oligodeoxynucleotide type B-2006-G5 (InvivoGen, CA) at 37 ° C in a humidified atmosphere containing 5% CO 2 .

    Techniques: Cell Culture, Staining